Senior Scientist

Location: Ridgefield, CT 06877

Duration: 12 Months

Note: Must have 2 years of Crispr experience. Molecular biology, qPCR, western blot. Proficiency in cell culture. Primary cell lines. Design guide RNA for a target, order a guide RNA, do the cell culture, grow cells, they do the electroporation, coordinate CRO work, submit samples on behalf of other Tas. They teach them single-cell-seq Every day will be cell culture work; QPCR, western blot If you have candidates who are willing to relocate at their own expense, we are willing to consider them as well.

Job Duties: Design, develop, and execute functional genomics assays, including CRISPR-based editing (e.g., RNP delivery, Perturb-seq), single-cell sequencing, spatial transcriptomics, and CITE-seq. Provide technical guidance and training to research scientists on FGSL technologies and techniques, including assay execution, troubleshooting, and resolution of technical issues; Documents and maintains detailed laboratory records in accordance with policies; Operates and maintains lab equipment in collaboration with other lab members; Assists acquisition of necessary supplies and equipment; Defines and performs appropriate QC measures; Follows relevant scientific literature to ensure use of optimal methods and understand emerging practices across the field; Demonstrates the ability to interpret the outcome of experiments, propose appropriate follow-up, and may propose new avenues of investigation; Communicates own work effectively orally and in writing; contributes to writing protocols, procedures, and technical reports Automate processing and results reporting and delivery; Reports and treats data with a high level of integrity and ethics.

Skills: CRISPR assays - Must have personally designed guides, performed transfections/infections, selected edited cells, and validated knockouts/knockins Next-generation sequencing (NGS) - Hands-on library preparation experience (not just data analysis)Single cell technologies - Personal experience with sample prep, library generation, and quality control Standard molecular biology techniques - qPCR, Western blot, cloning, gel electrophoresis Cell culture - Proficient in maintaining multiple cell lines, transfections, and sterile technique Experience in automation is a plus; Able to perform variety of FGS experimental techniques at the same time; Demonstrated experience working in a multi-disciplinary and collaborative environment; Ability to troubleshoot FGS experiments both individually and as part of a team. Excellent oral and written skills with the ability to communicate in an open, transparent, timely and consistent manner.

Education: Master’s degree from an accredited institution with 4+ years of experience in molecular, cell biology, or related scientific discipline