Laboratory Technician

Position Overview: The Xu Lab, in the Department of Biomedical Sciences at Marshall University's Joan C. Edwards School of Medicine, is seeking a part-time technician. The primary objective of this position is to construct genetic mutants of Streptococcus pyogenes.

Key Responsibilities: Mutant Construction: Design and generate targeted gene mutations in streptococcal strains. Molecular Cloning: Perform PCR, restriction cloning, plasmid purification, and gel electrophoresis. Bacterial Transformation: Introduce recombinant DNA into bacteria using electroporation or chemical competence. Strain Verification: Validate mutant strains using screening PCR and DNA sequencing data analysis. Inventory Tracking: Maintain clean records of primers, plasmids, and newly created glycerol stocks.

Location: MUSOM - Marshall University School of Medicine

Salary Range: Salary is commensurate with experience and internal equity

Education: Bachelors Degree in Microbiology, Molecular Biology, Biochemistry, or a related biomedical field

Experience: Prior hands-on experience with molecular cloning protocols is highly preferred. Core Skills: Proficiency in aseptic technique, bacterial culturing, and standard PCR assays. Attributes: Strong attention to experimental detail and reliable data documentation habits.